Dissociative mechanism for irreversible thermal denaturation of oligomeric proteins

Irreversible thermal denaturation of Glucose oxidase from Aspergillus niger

Thermal Denaturation Equilibria of Tmv Coat Proteins

Thermal denaturation of RNA free coat proteins of tobacco mosaic virus (TMV) was studied for wildtype TMV (vulgare) and the temperature-sensitive mutant, Ni 118. The ability to form soluble aggregates as well as the optical properties (ORD, UV-difference spectra), and the sedimen­ tation behavior were used as criteria for the native state. At pH 7.5 , 7 = 0 .0 2 denaturation is reversible for b...

Analysis of differential scanning calorimetry data for proteins. Criteria of validity of one-step mechanism of irreversible protein denaturation.

We consider in this work the analysis of the excess heat capacity C(p)(ex) versus temperature profiles in terms of a model of thermal protein denaturation involving one irreversible step. It is shown that the dependences of ln C(p)(ex) on 1 T (T is the absolute temperature) obtained at various temperature scanning rates have the same form. Several new methods for estimation of parameters of the...

Possible mechanism for cold denaturation of proteins at high pressure.

We study cold denaturation of proteins at high pressures. Using multicanonical Monte Carlo simulations of a model protein in a water bath, we investigate the effect of water density fluctuations on protein stability. We find that above the pressure where water freezes to the dense ice phase (approximately 2 kbars) the mechanism for cold denaturation with decreasing temperature is the loss of lo...

Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli.

Maltodextrin phosphorylase from Escherichia coli (MalP) is a dimeric protein in which each approximately 90-kDa subunit contains active-site pyridoxal 5'-phosphate. To unravel factors contributing to the stability of MalP, thermal denaturations of wild-type MalP and a thermostable active-site mutant (Asn-133-->Ala) were compared by monitoring enzyme activity, cofactor dissociation, secondary st...